They protect the key cell components by neutralizing the damaging effects of. In vitro antioxidant and antibacterial activity of. In vitro antioxidant methods, cellular antioxidant activity, cellular antioxidant activity, semi quantitative analysis, folinciocalteu method. The first group was composed by five substances with higher values of antioxidant activity. In vitro antioxidant activity and free radical scavenging. In vitro bioaccessibility and antioxidant activity of. Invitro antioxidant methods, cellular antioxidant activity, cellular antioxidant activity, semi quantitative analysis, folinciocalteu method. The present work is to investigate the in vitro hepatoprotective and antioxidant activity of ethanol extract of stem of g. Dpph method is the most frequently used one for in vitro antioxidant activity evaluation while lpo was found as the mostly used in vivo. The various antioxidant activities were compared with. Aug 01, 2009 the antimicrobial and antioxidant activity depended on the concentration and chemical nature of the phenolic compounds in the extracts. Bha was used as a standard antioxidant for dpph radical scavenging activity. In vitro evaluation on antioxidant and antimicrobial activity of spice extracts of ginger, turmeric and garlic virendra v. Phytochemical screening and in vitro antioxidant activity.
Comparing to trolox, pca shows much more effective antioxidant activity in vitro in both lipid and aqueous media. It is also possible to use screening methods to identify the class of antioxidant e. Pdf recent applications for in vitro antioxidant activity assay. To isolate and analyse the chemical composition in the essential oils and free radical scavenging activity of different crude extracts from the fresh and dry leaves of vegetable plants of lactuca sativa l. Cordia macleodii, an important medicinal plant traditionally used for the treatment of various disorders, including wound healing, jaundice, gastric ulcer, hepatoprotective activity and antivenom potential, thus the plant showed the high antioxidant. Grampositive bacteria were more sensitive than were gramnegative bacteria, especially for oilsoluble extracts with carnosic acid as. In vitro antioxidant activity of bixa orellana annatto seed.
Hptlc was performed on 10 cm x 10 cm aluminum backed plates coated with 0. Comparison of in vitro antioxidant activity of infusion. Jayaprakasha human resource development, central food technological research institute, mysore 570 0, india, and government college of pharmacy, bangalore 560 027, india. International journal of pharmacognosy and phytochemical research. Antioxidant activity of oat bran hydrolyzed proteins in vitro. Pdf in vitro antioxidant and antiproliferative activity of. Thirtyfour strains of lactic acid bacteria seven bifidobacterium, 11 lactobacillus, six lactococcus, and 10 streptococcus thermophilus were assayed in vitro for antioxidant activity against ascorbic and linolenic acid oxidation taaaa and taala, troloxequivalent antioxidant capacity teac, intracellular glutathione tgsh, and superoxide dismutase sod. Pdf antioxidant activity of petroleum ether, benzene, ethyl acetate, methanol and ethanol extracts of the whole plant of polycarpaea corymbosa have. In vitro antiadenovirus activity, antioxidant potential. Coffee is wrongly identified as only a stimulant because of its caffeine content. Phytochemical screening and in vitro antioxidant activity of ethyl acetate leaf extracts of pterocarpus marsupium roxb fabaceae kumaravel br. Dpph radical scavenging activity of different fruit extracts of r. In vitro antioxidant activity and in vivo efficacy of topical.
Screening of in vitro antioxidant activity of methanolic. In vitro antioxidant activity of the plant extracts was observed by dpph radical scavenging assay and reducing power methods. They protect the key cell components by neutralizing the. Toxicity and antioxidant activity in vitro and in vivo of two. The extract exhibited outstanding scavenging effect as evidenced from the study. Presently, 19 in vitro and 10 in vivo methods are being used for antioxidant evaluation purpose. This is a good study in which the authors have evaluated the in vitro antioxidant activity of the methanol extract of a. In vitro antioxidant activity and in vivo efficacy of. The present study was designed to evaluate the free radical scavenging activity of ethanol extract of leaf and stem of grewia carpinifolia using various in vitro. Recent applications for in vitro antioxidant activity assay.
Studies on the antioxidant activity of pomegranate punicagranatum peel and seed extracts using in vitro models r. Feb 19, 20 in vitro study on the antioxidant activity of a polyphenolrich extract from pinus brutia bark and its fractions elena cretu, 1 maarit karonen, 2 juhapekka salminen, 2 cornelia mircea, 3 adriana trifan, 1 christiana charalambous, 4 andreas i. Hence, the present study has been undertaken to investigate the antioxidant activity of the leaves of annona muricata with reference to the two other species of annona i. The white and black varieties of sesamum indicum were extracted in ethanol and the extracts were assayed for their antioxidant activities. Phytochemical investigation and in vitro antioxidant activity.
And in practice several in vitro test procedures are carried out for evaluating antioxidant activities with the samples of interest. Two extracts from the brown seaweed fucus vesiculosus containing 28. In vitro antioxidant activity and free radical scavenging potential of roots of malawian trichodesma zeylanicumm burm. Pdf on apr 9, 2014, sangh partap and others published in vitro antioxidant activity of whole plant of leptadenia pyrotechnica find. To evaluate and compare in vitro antioxidant activity of methanol extract of the indigenous withania somnifera with the imported root extract. The antioxidant activity of medicinal plants was at par with the commercial. The antioxidant content of coffee and its in vitro activity as an effect of its production method and roasting and brewing time by maciej gorecki and ewelina hallmann institute of human nutrition sciences, department of functional and organic food, warsaw university of life sciences, nowoursynowska 159c, 02776 warsaw, poland. Whereas there is slightly difference in activities as measured by tba method. A comparative study on in vitro antioxidant activity of the legumes. In vitro antioxidant activity and phenolic content of. Senthil kumarc abharathiar university, coimbatore, india bdepartment of biotechnology, sri krishna arts and sciene college, coimbatore641 008, india. Nov 17, 2001 antioxidantrich fractions were extracted from pomegranate punica granatum peels and seeds using ethyl acetate, methanol, and water.
Lemon balm extract mohammadtaghi moradi1, ali karimi2, somayeh alidadi3, leila hashemi4 1medical plants research center, shahrekord university of medical science, shahrekord, iran. In vitro antioxidant activity and phenolic content of croton. The antioxidant activity of grewia carpinifolia extract may be due to the high level of flavonoids and phenols in the plant. Inhibition of heme oxygenase antioxidant activity exacerbates. Invitro antioxidant and free radical scavenging activity.
These fruits contain ascorbic acid and polyphenols like gallic acid and ellagic acid which are responsible for its antioxidant activity. Studies on the antioxidant activity of pomegranate punica. In vitro antioxidant activity of bixa orellana annatto seed extract 1 modupeola abayomi, 2 amusa s. The antioxidant activity of plant extracts was determined by different in vitro methods such as the dpph free radical scavenging assay and reducing power methods. Panpatil, shalini tattari, nirmala kota, chetan nimgulkar, kalpagam polasa abstract spices like turmeric, ginger, alliums are indispensable for the preparation of our daily food and are reported. In vitro antimicrobial and antioxidant activity of commercial. In vitro antioxidant and antibacterial activity of endophytic fungi isolated from mussaenda luteola shylaja gunasekaran, mythili sathiavelu, sathiavelu arunachalam. Protocatechuic acid pca is a natural phenolic acid widely distributed in plants and is considered as an active component of some traditional chinese herbal medicines such as cibotium barometz l. The antioxidant content of coffee and its in vitro activity.
In vitro antioxidant activity of vetiveria zizanioides. Evaluation of the in vitro and in vivo antioxidant. Respect to its abilility in inhibiting the lipid peroxidation. The first part of this thesis focusses on the activity of proteins extracted in the presence of salt and digested with trypsin tph and alcalase aph. Antioxidant activity by dpph assay of potential solutions. In vitro antioxidant activity in vitro antioxidant activity of isolated compounds was investigated using dpph free radical scavenging assay with slight modifications amin, norazaidah, emmy, 2006. In vitro antioxidant and antibacterial activity of lamiaceae phenolic extracts.
Aa and its derivates presented an in vitro antioxidant activity but aa had the best antioxidant effect. Jul 12, 2012 thirtyfour strains of lactic acid bacteria seven bifidobacterium, 11 lactobacillus, six lactococcus, and 10 streptococcus thermophilus were assayed in vitro for antioxidant activity against ascorbic and linolenic acid oxidation taaaa and taala, troloxequivalent antioxidant capacity teac, intracellular glutathione tgsh, and superoxide dismutase sod. The methanol extract of peels showed 83 and 81% antioxidant activity at 50. The digests tph and aph were ultrafiltered using 2kda, and 10kda molecular cutoff membranes. Plant derived antioxidant compounds, flavonoids and phenolics have received considerable attention because of their physiological effect like antioxidant, antiinflammatory, antitumor activities and low.
Ethiopia is one of the developing countries which have enormous diversity of plants. For example, tlc screening may be used10,11 to identify components in extracts that exhibit such activity. Furthermore a positive correlation has been found between the frap assay, phosphomolybdenum test and sod, catalase, peroxidase activity assays table 8 demonstrating that the increase of the antioxidant potential is proportionally related to the increase in the activity of antioxidant enzymes in vitro and thus a decrease in the free radicals. In vitro study on the antioxidant activity of a polyphenol. The different extracts were dissolved in ethanol at a concentration of 50200 mgml. Invitro antioxidant and free radical scavenging activity of bauhinia variegata linn sonam pandey, r. In vitro antioxidant activity of methanolic and aqueous. In vitro antiadenovirus activity, antioxidant potential and. In vitro screening for anticholinesterase and antioxidant. Pdf in vitro antioxidant and antibacterial activity of.
In vitro antioxidant activity of valeriana officinalis. Antioxidant activity of oat bran hydrolyzed proteins in. Antioxidantrich fractions were extracted from pomegranate punica granatum peels and seeds using ethyl acetate, methanol, and water. Benisuef university journal of basic and applied sciences. The antioxidant activity measured at the end of the digestion procedure was 35. Antioxidant assay using acarotenelinoleate model system. The study revealed that both the extracts showed antioxidant activity. Dpph scavenging activities were assayed principally according to dzamic et al. The antioxidant activity of pomegranate peel and seed extracts was evaluated according to the method of jayaprakasha et al. The high production and health properties of coffee make it one of the best among daily drinks. Hence, in the present investigation various extracts of s. The in vitro antioxidant assay of ame revealed that it has a potent antioxidant activity comparable to vitamin c which was used as a reference standard. The consumption of seaweeds has increased in recent years.
Antioxidant activity invitro antioxidant studies are widely carried to screen various plant containing phenolic and flavanoids constituents. Frank ngonda department of biological sciences, chancellor college, university of malawi, p. Reviews on mechanisms of in vitro antioxidant activity of polysaccharides article pdf available in oxidative medicine and cellular longevity 201664. In vitro antioxidant activity of bixa orellana annatto. Vitamin c is used as a dietary supplement because of its antioxidant activity, although a high dose 500 mg may act as a prooxidant in the body1,2. A report on the in vitro antioxidant properties of vachellia karroo. The hydroxyl radical scavenging by the white sesame extract was found to be more than that of black sesame. Coffee is one of the most popular beverages in the world.
Antioxidant activity and mechanism of protocatechuic acid in. A correlation study ivana generali mekini 1, danijela skroza 1, ivica ljubenkov 2, v ida imat 3. Grampositive bacteria were more sensitive than were gramnegative bacteria, especially for oilsoluble extracts with carnosic acid as the major phenolic compound. Because most natural antioxidants and phytochemicals are multifunctional i. Pdf in vitro antioxidant and antiproliferative activity. Reviews on mechanisms of in vitro antioxidant activity of. For preliminary screening of the sample, the antioxidant activity was evaluated by using hptlcdpph bioautography method 7, 8. Antioxidant properties of potentially probiotic bacteria. Review on in vivo and in vitro methods evaluation of.
Vitro bioactivities of the extracts of aerial part of evolvulus nummularius. Pdf chemical composition of essential oils and in vitro. Maheshwari research department, jawaharlal nehru cancer hospital and research centre, bhopal, m. Methanolic extracts of nelumbo nucifera, punica granatum and rauvolfia serpentina also showed high antioxidant activity with ic 50 values 453. Introduction a antioxidant is a chemical that prevents the oxidation of other chemicals. In vitro antioxidant studies in leaves of annona species 481 are available to show the antioxidant property of the plant with relation to its anticancer activity.
The reducing power assay of extracts was carried out with ascorbic acid as a. The total phenolic content and antioxidant activities of acacia auriculiformis, acacia ferruginea and cajanus cajan seed extracts were examined. Jun 22, 2000 vitamin c is used as a dietary supplement because of its antioxidant activity, although a high dose 500 mg may act as a prooxidant in the body1,2. In vitro potential antioxidant activity of 1 3, 1 6. School of pharmacy, college of health sciences, mekelle university 1, mekelle, ethiopia. To evaluate the antioxidant potential and free scavenging activity of t. Evaluation of the in vitro and in vivo antioxidant potentials. The antimicrobial and antioxidant activity depended on the concentration and chemical nature of the phenolic compounds in the extracts. Hence, it could therefore be used in pharmacological or food industry as a natural antioxidant. In vitro antioxidant activity the free radical scavenging activity of the methanolic leaf and root extracts of the study species, h. In the present study, the antioxidant activity of ppe was determined using different assay systems.
Antioxidant activity should not be concluded based on a single antioxidant test model. Antioxidant activity and mechanism of protocatechuic acid in vitro background. In vitro antioxidant activity and total phenolic content. Gnetum ula is a notable medicinal plant used to cure various ailments. Adebayo, 3 deon bennett, 4 roy porter, and 1 janet shellycampbell 1 school of pharmacy, college of health sciences, university of technology, jamaica, 237 old hope road, kingston 6. Briefly, testing samples of ce, hf, mf, eaf, eafsub, compounds 1, 2 and 3 were prepared in various concentrations 20, 40, 60, 80 and 100. In vitro antimicrobial and antioxidant activity of. In vitro antioxidant activity of withania somnifera root. On the other hand, coffee is one of the best sources of other bioactive compounds, such as flavonoids and phenolic acids. The stem part of the plant is used traditionally to treat jaundice and other disorders. In vitro antioxidant properties of antioxidant properties of antioxidant. The extracts showed potent antioxidant activity in all models when compared with ascorbic acid having ic values 8.
The antioxidant content of coffee and its in vitro. The substances can be divided in three main groups, as depicted in table 1. In vitro antioxidant activity of rubus ellipticus fruits ncbi. The free radical scavenging activity of the fractions was measured in vitro by 1, 1diphenyl2picrylhydrazyl dpph assay. In in vivo efficacy studies, only the formulation containing aa caused alterations in tewl values and the formulation containing map caused alterations in the viscoelastic. In vitro antiadenovirus activity, antioxidant potential and total phenolic compounds of melissa officinalis l. Ic values of methanolic and aqueous extracts were found 50 to be 18 gml and 26 gml for dpph and 62 gml 75 gml for nitric oxide scavenging activity. Rapid screening for in vitro antioxidant activity by hptlcbioautography method. Valerian is widely used as a traditional medicine to improve the quality of sleep.
Investigation on the in vitro antioxidant capacity of. This study shows that the endophytic fungi isolated m. Nafld patients are at risk of progressing to nonalcoholic steatohepatitis nash, associated to cardiovascular diseases cvd, coronary heart disease and stroke. Antioxidant activity and mechanism of protocatechuic acid. The various antioxidant activities were compared with suitable antioxidants such as butyl hydroxy toluene. In vitro antioxidant activity assay in vitro antioxidant activity of the infusion, e extract and its fractions were assayed by using dpph scavenging method. The progression of nonalcoholic fatty liver disease nafld and the development of hepatic fibrosis is caused by changes in redox balance, leading to an increase of reactive oxygen species ros levels. Methanolic extracts of nelumbo nucifera, punica granatum and rauvolfia serpentina also showed high antioxidant activity with ic 50.
Another aspect is that antioxidant test models vary in different respects. In vitro antioxidant activity of vetiveria zizanioides root. In vitro antioxidant activity, total phenolic and flavonoid contents of. Abstract identification and characterization of natural products with antioxidant and antiproliferative activity has received much interest over the past few years. Screening of in vitro antioxidant activity of methanolic leaf. In vitro antioxidant activity of rubus ellipticus fruits. Thereafter, the effects of ppe in modulating the production of proin. However, their adverse and beneficial effects have scarcely been studied. The extracts were screened for their potential as antioxidants using various in vitro models, such as. In vitro bioaccessibility and antioxidant activity of grape.
851 1479 1527 632 1508 212 675 135 369 1027 301 1441 1491 1353 140 114 272 1047 1105 30 594 23 385 322 696 527 1236 1483 1058 25 184 340 714 391